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| Oliveira,A; Cunha,MLRS. |
| In addition to their capacity to attach to surfaces, various groups of microorganisms also produce an extracellular polymeric substance known as "slime". This slime forms a thin layer around cells known as biofilm. Thus, biofilm structure comprises bacterial cells and an extracellular polymeric substance. It also presents a defined architecture, providing the microorganisms with an excellent protective environment and favoring the exchange of genetic material between cells as well as intercellular communication. The ability to produce biofilm is observed in a large group of bacteria, including coagulase-negative staphylococci (CNS) which are the predominant microorganisms of normal skin flora and have been implicated as the causative agents of hospital... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative staphylococci; Biofilm; Slime; Infection; Microbiology. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400003 |
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| Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap. |
| This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009 |
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| Peña,Joan; Uffo,Odalys. |
| Staphylococcus aureus es uno de los patógenos más importante de la mastitis bovina. La producción de biofilme por parte de las bacterias asociadas a las infecciones intramamarias, les confiere mayor capacidad de colonizar el tejido y de protección contra el sistema inmune y contra la acción de antimicrobianos. En este estudio, se trabajó con 98 aislados cubanos de S. aureus, previamente genotipados para la región X de la proteína A, colectados de cinco provincias de Cuba y se testaron para la producción de slime y biofilme. Se observó que el 98% de los aislados tuvieron algún tipo de producción de biofilme. Se considera que el método por tinción con Violeta Cristal utilizando placas de poliestireno es el método de elección, pues el método de producción de... |
| Tipo: Journal article |
Palavras-chave: Biofilme; Slime; Staphylococcus aureus; Mastitis bovina; Genotipo Spa. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000300007 |
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| Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de. |
| Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010 |
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